Inkcazo eneenkcukacha
I-Canine parvovirus antigen ikiti yovavanyo olukhawulezayo isebenzisa umgaqo wendlela yesandwich ephindwe kabini ye-antibody ukubona ngokusemgangathweni i-canine parvovirus antigen kwilindle lezinja.Umgangatho wegolide we-dog parvovirus antibody 1 wasetyenziswa njengesiphawuli sesalathisi, kwaye ummandla wokufumanisa (T) kunye nommandla wolawulo (C) kwinwebu ye-nitrocellulose yaqatywa nge-canine parvovirus antibody 2 kunye ne-anti-chicken yeegusha, ngokulandelanayo.Ngexesha lokufumanisa, isampuli i-chromatographic phantsi kweempembelelo ze-capillary.Ukuba isampuli evavanyiweyo iqulethe i-canine parvovirus antigen, i-antibody esemgangathweni ye-1 yenza i-antigen-antibody complex kunye ne-canine parvovirus, kwaye idibanisa ne-canine parvovirus antibody 2 egxininiswe kwindawo yokubona ngexesha le-chromatography ukwenza "i-antibody 1-antigen-antibody 2" isangweji. , okubangelwa ibhendi ebomvu ebomvu kwindawo yokufumanisa (T);Ngokuchaseneyo, akukho zibhendi ezimfusa-bomvu zivela kwindawo yokufumanisa (T);Kungakhathaliseki ukuba khona okanye ukungabikho kwe-canine parvovirus antigen kwisampuli, i-IgY complex yenkukhu esemgangathweni yegolide iya kuqhubeka ibekwe phezulu ukuya kwindawo yokulawula (C), kwaye ibhendi ebomvu-purplish iya kuvela.Ibhendi emfusa-bomvu enikezelwe kwindawo yolawulo (C) ngumgangatho wokugweba ukuba inkqubo yechromatography iqhelekile, kwaye ikwasebenza njengomgangatho wolawulo lwangaphakathi kwii-reagents.
Imixholo eyenzelwe wena
