ISISHWANKATHELO NENKCAZO UVAVANYO
Iintsholongwane zeDengue, usapho lwee-serotypes ezine ezihlukeneyo zentsholongwane (i-Den 1,2,3,4), i-singlestrained, i-enveloped, i-virus ye-RNA enengqiqo.Ezi ntsholongwane zisasazwa ziingcongconi zosapho lwakwaStegemyia oluluma emini, ingakumbi iAedes aegypti, kunye neAedes albopictus.Namhlanje, abantu abangaphezu kwe-2,5 bhiliyoni abahlala kwimimandla ekweleenjiko yaseAsia, eAfrika, eOstreliya nakumazwe aseMerika basesichengeni sokwasulelwa yidengue.Kuqikelelwa ukuba i-100 lezigidi zeemeko zomkhuhlane wedengue kunye neemeko ezingama-250,000 zedengue ebeka ubomi esichengeni umkhuhlane owophayo zenzeka minyaka le kwihlabathi liphela1-3.
Ukufunyanwa kwe-serological ye-antibody ye-IgM yeyona ndlela ixhaphakileyo yokuxilongwa kosulelo lwentsholongwane yedengue.Kutshanje, ukufunyanwa kwee-antigens ezikhutshwe ngexesha lokuphindaphinda kwentsholongwane kwisigulana esosulelekileyo kubonise iziphumo ezithembisayo.Ivumela ukuxilongwa ukususela ngosuku lokuqala emva kokuqala komkhuhlane ukuya kumhla we-9, emva kokuba isigaba sekliniki sesi sifo siphelile, ngaloo ndlela ivumela unyango lwakwangoko lubekwe ngokukhawuleza4-.Uvavanyo olukhawulezayo lweDengue IgG/IgM luphuhliswa ukuze lubone ukujikeleza kwe-antigen yedengue. kwiserum, kwiplasma okanye kwigazi elipheleleyo.Uvavanyo lunokwenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwezixhobo zaselabhoratri.
UMGAQO
Uvavanyo lweDengue IgG/IgM oluKhawulezayo luvavanyo lwe-chromatographic immunoassay olusecaleni.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equlethe i-dengue recombinant envelope antigens ehlanganiswe negolide ye-colloid (i-dengue conjugates) kunye nomvundla we-IgG-gold conjugates, 2) i-nitrocellulose inwebu equlethe iibhendi ezimbini zovavanyo (i-G kunye ne-M bands) kunye ibhendi yolawulo (C band).I-G band ifakwe ngaphambili kunye ne-antibody ukuze kubonwe intsholongwane ye-IgG yokulwa ne-dengue, i-M band iqatywe nge-antibody ukuze ibonwe i-IgM yokulwa ne-virus ye-dengue, kwaye i-C band ifakwe ngaphambili nge-igG yebhokhwe yokulwa nomvundla.
Xa umthamo owaneleyo wovavanyo lovavanyo lukhutshwa kwisampulu yequla lekhasethi yovavanyo, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.Intsholongwane ye-IgG echasene nedengue ukuba ikhona kumzekelo iya kubophelela kwiiconjugates zedengue.I-immunocomplex iye ibanjwe yi-reagent egqunywe kwi-G band, yenza i-burgundy enemibala ye-G band, ebonisa i-virus ye-dengue IgG isiphumo sovavanyo olulungileyo kwaye iphakamisa usulelo lwamva nje okanye oluphindayo.Intsholongwane ye-IgM echasene nedengue, ukuba ikhona kumzekelo, iya kubophelela kwi-conjugates yedengue.I-immunocomplex iye ibanjwe yi-reagent efakwe ngaphambili kwi-M band, eyenza i-burgundy enemibala ye-M band, ebonisa i-virus ye-dengue IgM isiphumo sovavanyo olulungileyo kwaye iphakamisa usulelo olutsha.
Ukungabikho naziphi na iibhendi zovavanyo (G kunye M) icebisa isiphumo esibi.Uvavanyo luqulathe ulawulo lwangaphakathi (C band) ekufuneka ibonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG/mvundla IgG-gold conjugate kungakhathaliseki umbala. uphuhliso kuwo nawaphi na amanqwanqwa e-T.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
