ISISHWANKATHELO NENKCAZO UVAVANYO
I-Leptospirosis yenzeka kwihlabathi jikelele kwaye yingxaki eqhelekileyo kwimpilo yabantu nakwizilwanyana, ngakumbi kwiindawo ezinemozulu eshushu nefumileyo.Amadama endalo eleptospirosis ziimpuku kunye neentlobo ngeentlobo zezilwanyana ezanyisayo ezifuywayo.Ukusuleleka kwabantu kubangelwa yi-L. interrogans, ilungu le-pathogenic ye-genus ye-Leptospira.Usulelo lusasazwa ngomchamo wesilwanyana eso.
Emva kosulelo, iileptospires zikhona egazini de zicocwe emva kweentsuku ezi-4 ukuya kwezi-7 emva kokuveliswa kwe-anti-L.i-interrogans antibodies, ekuqaleni kweklasi ye-IgM.Inkcubeko yegazi, umchamo kunye ne-cerebrospinal fluid yindlela efanelekileyo yokuqinisekisa ukuxilongwa ngexesha le-1 ukuya kwiiveki ze-2 emva kokuvezwa.Ukufunyanwa kwe-serological ye-antiL.I-interrogans antibodies ikwayindlela eqhelekileyo yokuxilonga.Iimvavanyo zifumaneka phantsi kolu didi: 1) Uvavanyo lwe-microscopic agglutination (MAT);2) ELISA;3) Iimvavanyo ze-antibody ze-fluorescent ezingathanga ngqo (IFATs).Nangona kunjalo, zonke iindlela ezichazwe ngasentla zifuna indawo ephucukileyo kunye neengcali eziqeqeshwe kakuhle.
I-Leptospira IgG/IgM luvavanyo olulula lwe-serological olusebenzisa ii-antigens ezivela kwi-L. interrogans kwaye lubhaqa ii-antibodies ze-IgG kunye ne-IgM kwezi microorganisms ngaxeshanye.Uvavanyo lunokwenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwesixhobo sebhubhoratri esinzima kwaye iziphumo ziyafumaneka kwimizuzu eli-15.
UMGAQO
ILeptospira IgG/IgM Rapid Test Kit yi-lateral flow chromatographic.
uvavanyo lwe-immunoassay.Ikhasethi yovavanyo iquka: 1) i-burgundy colored conjugate pad equkethe i-recombinant L. interrogans antigens conjugated negolide ye-colloid (i-Leptospira conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocellulose inwebu equlethe iibhendi ezimbini zokuvavanya (i-M kunye ne-G bands) kunye nebhendi yokulawula (i-C band).I-M band ifakwe ngaphambili kunye ne-monoclonal anti-human IgM yokukhangela i-anti-L.. i-interrogans IgM, i-G band ifakwe ngaphambili kunye ne-reagents yokukhangela i-anti-L.interrogans IgG, kunye C band pre-uqatywe ngebhokhwe anti umvundla IgG.
Xa umthamo owaneleyo wesampulu yovavanyo ukhutshwa kwisampulu yequla lekhasethi, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.I-IgM anti-L.. i-interrogans ukuba ikhona kwi-specimen iya kubophelela kwi-conjugates ye-Leptospira.I-immunocomplex iye ibanjwe kwi-membrane yi-antibody ye-IgM efakwe ngaphambili, eyenza i-burgundy enemibala ye-M band, ebonisa i-L.. interrogans IgM isiphumo sovavanyo oluhle.I-IgG anti-L.. i-interrogans ukuba ikhona kwi-specimen iya kubophelela kwi-conjugates ye-Leptospira.I-immunocomplex ke ibanjwe yi-reagents efakwe ngaphambili kwi-membrane, yenza i-burgundy enemibala ye-G band, ebonisa i-L.. interrogans IgG isiphumo sovavanyo oluhle.
Ukungabikho kwawo nawaphi na amabhanti ovavanyo (M no-G) acebisa isiphumo esibi.Uvavanyo iqulathe ulawulo lwangaphakathi (C band) ekufuneka abonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG / umvundla IgG-igolide conjugate kungakhathaliseki ukuba uphuhliso umbala kuyo nayiphi na bands uvavanyo.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
