ISISHWANKATHELO NENKCAZO UVAVANYO
Isifo seengcongconi sisifo seengcongconi, esosulelayo, esosulela abantu abangaphezu kwezigidi ezingama-200 kwaye sibulala abantu abangaphezu kwesigidi esi-1 ngonyaka.Ibangelwa ziindidi ezine zePlasmodium: P. falciparum, P. vivax, P. ovale, kunye neP. malariae.Ezi plasmodia zosulela kwaye zitshabalalise iierythrocyte zabantu, zivelisa iingqele, umkhuhlane, ianemia, kunye nesplenomegaly.I-P. falciparum ibangela isifo esibi kakhulu kunezinye iintlobo ze-plasmodial kwaye ibangela uninzi lokufa kwemalariya.I-P. falciparum kunye ne-P. vivax zezona zifo zixhaphakileyo, nangona kunjalo, kukho ukuhluka okukhulu kwejografi ekusasazeni iintlobo.
Ngokwemveli, isifo seengcongconi sifunyaniswa ngokuboniswa kwezinto eziphilayo kwi-Giemsa ene-smears eshinyeneyo yegazi le-peripheral, kwaye iintlobo ezahlukeneyo ze-plasmodium zahlulwa ngokubonakala kwazo kwii-erythrocyte ezosulelekileyo.Ubuchwephesha buyakwazi ukuxilonga ngokuchanekileyo nangokuthembekileyo, kodwa kuphela xa kusenziwa zii-microscopists ezinezakhono zisebenzisa iiprothokholi ezichaziweyo, ezibonisa imiqobo emikhulu kwiindawo ezikude nezihluphekayo zehlabathi.
Uvavanyo lweMalaria Pf/Pv Ag Rapid Test luphuhliselwe ukusombulula le miqobo.Isebenzisa ii-antibodies ezikhethekileyo kwi-P. falciparum Histidine Rich Protein-II (pHRP-II) kunye ne-P. vivax Lactate Dehydrogenase (Pv-LDH) ukufumanisa kunye nokwahlula usulelo nge-P. falciparum kunye ne-P. vivax.Uvavanyo lunokwenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwezixhobo zaselabhoratri
UMGAQO
UVavanyo oluKhawulezayo lweMalaria Pf/Pv Ag luvavanyo lwe-chromatographic flow lateral.Amacandelo ovavanyo lwe-strip aquka: 1) i-burgundy colored conjugate pad equlethe i-anti-Pv-LDH ye-antibody edityaniswe ne-colloid yegolide (i-Pv-LDH-igolide ye-conjugates) kunye ne-mouse echasene ne-pHRP-II i-antibody ehlanganiswe negolide ye-colloid (pHRP-II). -i-conjugates yegolide), i-2) i-membrane ye-nitrocellulose eneebhendi ezimbini zokuvavanya (i-Pv kunye ne-Pf bands) kunye nebhendi yokulawula (i-C band).Ibhendi ye-Pv ifakwe ngaphambili kunye nenye i-anti-Pv-LDH ye-anti-Pv-LDH ekhethekileyo yokuchongwa kosulelo lwe-Pv, ibhendi ye-Pf ifakwe ngaphambili kunye ne-polyclonal anti-pHRP-II antibodies ukuze kufunyanwe usulelo lwe-Pf, kunye ne-C band. ngebhokhwe anti-mouse IgG.
Ngexesha lovavanyo, umthamo owaneleyo wesampuli yegazi unikezelwa kwisampulu kakuhle (S) yekhasethi yovavanyo, i-lysis buffer yongezwa kwi-buffer well (B).Isithinteli sinesixhobo sokucoca esilalisa iiseli ezibomvu zegazi kwaye sikhuphe ii-antigens ezahlukeneyo, ezithi zifuduke ngentshukumo yecapillary kumgca obanjwe kwikhasethi.I-Pv-LDH ukuba izipho kumzekelo ziya kubophelela kwi-conjugates ye-Pv-LDH-gold.I-immunocomplex iye ibanjwe kwi-membrane yi-anti-Pv-LDH antibody efakwe ngaphambili, eyenza i-burgundy enemibala ye-Pv band, ebonisa isiphumo sovavanyo lwe-Pv.Kungenjalo, i-pHRP-II ukuba iipresent in the specimen iya kubophelela kwi-pHRP-II-gold conjugates.I-immunocomplex ke ibanjwe kwi-membrane yi-anti-pHRP-II antibodies efakwe ngaphambili, eyenza i-burgundy enemibala ye-Pf band, ebonisa isiphumo sovavanyo oluhle lwe-Pf.Ukungabikho kwawo nawaphi na amabhanti ovavanyo kuphakamisa isiphumo esibi.Uvavanyo luqulathe ulawulo lwangaphakathi (C band) ekufuneka ibonise burgundy umbala band ye immunocomplex yebhokhwe anti-mouse IgG / mouse IgG (anti-Pv-LDH kunye anti-pHRPII) -conjugates zegolide kungakhathaliseki uphuhliso umbala kuyo nayiphi na. yamaqela ovavanyo.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
