Qrf Antibody Test Iphepha elinganqunyulwanga

Qrf Uvavanyo lwe-Antibody ye-Qrf Iphepha Elinganqunyulwanga Eliphawulweyo

Uvavanyo lwe-Qrf Antibody

Uhlobo: Iphepha elingasikwanga

Uphawu: Bio-mapper

Ikhathalogu: REA0911

Umzekelo: WB/S/P

Ikhithi yokuvavanya intsholongwane yerhashalala yegusha isetyenziselwa ukukhangela izithinteli-gazi zerhashalala kwiserum yeegusha, ezinokuthi zisetyenziswe ukuvavanya amandla omzimba wogonyo lwerhashalala okanye ukuncedisa ekuchongeni isifo.Umgaqo-siseko kukuba ikhithi yokubona i-antibody yegusha yepox iqulunqwe nge-protein ye-protein ye-nuclear ye-antigen plate egqunywe ngaphambili, iziphawuli ze-enzyme kunye nezinye ii-reagents ezixhasayo, kunye nomgaqo we-enzyme-linked immunoassay (ELISA) isetyenziselwa ukufumanisa irhashalala yegusha. amajoni omzimba kwisampulu yeserum yeegusha.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Inkcazo eneenkcukacha

Ukufunyaniswa kwe-antibody ye-virus ye-pox yegusha yenziwe nge-microplate efakwe ngaphambili kunye ne-protein ye-nyukliya ye-protein yegusha, iziphawuli ze-enzyme kunye nezinye ii-reagents ezixhasayo, kunye nomgaqo we-enzyme-linked immunoassay (ELISA) isetyenziselwa ukufumanisa i-antibody ye-pox yegusha kwindawo. isampuli serum yeegusha.Ngexesha lovavanyo, i-serum yolawulo kunye nesampuli ekufuneka ihlolwe yongezwa kwipleyiti ye-microplate, kwaye ukuba isampuli iqulethe i-antibody ye-pox yegusha emva kokufukanyelwa, iya kubotshwa kwi-antigen kwipleyiti ye-microplate, kunye namanye amacandelo angabotshwanga. iya kususwa emva kokuhlamba;Emva koko yongeza i-enzyme marker ukuba ibophe ngokuthe ngqo kwi-antigen-antibody complex kwi-microplate plate;Iimpawu ze-enzyme ezingabotshwanga zaye zasuswa ngokuhlamba, kwaye isisombululo se-TMB substrate songezwa emaquleni, kwaye imveliso eluhlaza okwesibhakabhaka yenziwa ngokusabela kwe-conjugates ye-microplate, kwaye ubunzulu bombala bunxulunyaniswe ngokufanelekileyo kunye nesixa esithile se-antibodies equlethwe kwi-antibody. isampuli.Emva kokuba isisombululo sokuphelisa songezwa ukuphelisa ukusabela, imveliso yajika yaphuzi;Ixabiso lokufunxa kwi-reaction nganye kakuhle lichongwa ngumfundi we-microplate kwi-wavelength ye-450 nm ukugqiba ukuba isampuli iqulethe ii-antibodies ze-pox yegusha.