ISISHWANKATHELO NENKCAZO UVAVANYO
Isifo sephepha sisifo esingapheliyo, esosulelayo esibangelwa ikakhulu yi-M. TB hominis (Koch's bacillus), ngamanye amaxesha yi-M. TB bovis.Eyona nto kujoliswe kuyo yimiphunga, kodwa naliphi na ilungu linokosuleleka.
Umngcipheko wosulelo lwe-TB wehle kakhulu kwinkulungwane yama-20.Nangona kunjalo, ukuvela kutsha nje kohlobo olunganyangekiyo ngamachiza1, ngakumbi phakathi kwezigulane ezine-AIDS2, kuvuselele umdla kwi-TB.Iziganeko zosulelo zixelwe malunga neemeko ezizizigidi ezisi-8 ngonyaka kunye nezinga lokufa kwezigidi ezi-3 ngonyaka.Ukusweleka kudlule kwi-50% kumazwe athile ase-Afrika anamazinga aphezulu e-HIV.
Ukurhanelwa kokuqala kwezonyango kunye nokufunyaniswa kweradiographic, kunye nokuqinisekiswa kwelebhu elandelayo ngovavanyo lwesikhohlela kunye nenkcubeko yindlela (iindlela) zesiNtu ekuxilongweni kwe-TB5,6 esebenzayo.Nakuba kunjalo, ezi ndlela mhlawumbi azinabuntununtunu okanye zitya ixesha, ngakumbi azifanelekanga izigulane ezingakwaziyo ukukhupha isikhohlela ngokwaneleyo, isikhohlela esingenayo, okanye ekurhaneleka ukuba sine-TB yangaphandle kwemiphunga.
I-TB IgG/IgM Combo Rapid Test iphuhliselwe ukunciphisa le miqobo.Uvavanyo lufumanisa i-IgM kunye ne-IgG ye-anti-M.TB kwi-serum, i-plasm, okanye igazi elipheleleyo kwi-15 imizuzu.Isiphumo esihle se-IgM sibonisa usulelo olutsha lwe-M.TB, ngelixa impendulo entle ye-IgG ibonisa usulelo oludlulileyo okanye olungapheliyo.Isebenzisa ii-antigens ezithile ze-M.TB, ikwabhaqa i-IgM i-anti-M.TB kwizigulana ezigonywe nge-BCG.Ukongeza, uvavanyo lunokuba
eyenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci ngaphandle kwesixhobo esinzima selabhoratri.
UMGAQO
UVavanyo oluKhawulezayo lwe-TB lwe-IgG/IgM luvavanyo lwe-chromatographic immunoassay olusecaleni.Ikhasethi yovavanyo iqukethe: 1) i-burgundy colored conjugate pad equlethe ii-antigens ze-M.TB ezidityaniswe negolide ye-colloid (i-M.TB conjugates) kunye nomvundla we-IgG-gold conjugates, i-2) i-nitrocellulose membrane strip equlethe iibhendi ezimbini zovavanyo (i-M kunye ne-G bands ) kunye nebhendi yolawulo (i-C band).I-M band ifakwe ngaphambili kunye ne-monoclonal anti-human IgM yokukhangela i-IgM ye-anti-M.TB, i-G band ifakwe ngaphambili kunye ne-reagents yokuchongwa kwe-IgG ye-anti-M.TB, kunye ne-C band ngaphambili. -igqunywe ngebhokhwe echasene nomvundla IgG.
Xa umthamo owaneleyo wesampulu yovavanyo ukhutshwa kwisampulu yequla lekhasethi, umzekelo ufuduka ngesenzo se-capillary kwikhasethi.I-IgM i-anti-M.TB ukuba ikhona kumzekelo iya kubophelela kwi-conjugates ye-M.TB.I-immunocomplex iye ibanjwe kwi-membrane yi-anti-human IgM antibody, eyenza i-burgundy band M enemibala, ebonisa isiphumo sovavanyo lwe-M.TB IgM.I-IgG i-anti-M.TB, ukuba ikhona kumzekelo, iya kubophelela kwi-conjugates ye-M.TB.I-immunocomplex ke ibanjwe yi-reagents efakwe ngaphambili kwi-membrane, yenza i-burgundy enemibala ye-G band, ebonisa isiphumo sovavanyo lwe-M.TB IgG.Ukungabikho kwawo nawaphi na amabhanti ovavanyo (M no-G) acebisa isiphumo esibi.Uvavanyo iqulathe ulawulo lwangaphakathi (C band) ekufuneka abonise band burgundy enemibala ye immunocomplex yebhokhwe anti umvundla IgG / umvundla IgG-igolide conjugate kungakhathaliseki uphuhliso lombala kuyo nayiphi na bands T.Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
