Malaria Pf/Pan Antigen Rapid Test kit (Colloidal Gold)

Isifo seengcongconi sisifo seengcongconi, esosulelayo, esosulela abantu abangaphezu kwezigidi ezingama-200 kwaye sibulala abantu abangaphezu kwesigidi esi-1 ngonyaka.Ibangelwa ziindidi ezine zePlasmodium: P. falciparum, P. vivax, P. ovale, kunye neP. malariae.Ezi plasmodia zosulela kwaye zitshabalalise iierythrocyte zabantu, zivelisa iingqele, umkhuhlane, ianemia, kunye nesplenomegaly.I-P. falciparum ibangela isifo esibi kakhulu kunezinye iintlobo ze-plasmodial kwaye ibangela uninzi lokufa kwemalariya.I-P. falciparum kunye ne-P. vivax zezona zifo zixhaphakileyo, nangona kunjalo, kukho ukuhluka okukhulu kwejografi ekusasazeni iintlobo.

Ngokwemveli, isifo seengcongconi sifunyaniswa ngokubonakaliswa kwezinto eziphilayo kwi-Giemsa ene-smears engqindilili yegazi le-peripheral, kwaye iintlobo ezahlukeneyo zeplasmodium zahlulwa ngokubonakala kwazo kwii-erythrocytes ezosulelekileyo1.Ubuchwephesha buyakwazi ukuxilonga ngokuchanekileyo nangokuthembekileyo, kodwa kuphela xa kusenziwa ziingcali zemakroskopu ezinezakhono zisebenzisa iiprothokholi ezichaziweyo2, ezibonisa imiqobo emikhulu kwiindawo ezikude nezihluphekayo zehlabathi.

I-Malaria Pf / Pan Antigen Rapid Test Kit iphuhliselwe ukusombulula le miqobo.Uvavanyo lusebenzisa iperi ye-monoclonal kunye ne-polyclonal antibodies kwi-P. falciparum protein ethile, i-Histidine Repeat Protein II (pHRP-II), kunye nepere ye-monoclonal antibodies kwi-plasmodium Lactate Dehydrogenase (pLDH), iprotheni eveliswa zintlobo ezine ze i-plasmodium, ngaloo ndlela yenza ukuba kubonwe ngaxeshanye kunye nokwahlulwa kosulelo nge-P. falciparum kunye okanye nayiphi na enye i-plasmodia emithathu.Ingenziwa ngabasebenzi abangaqeqeshwanga okanye abanezakhono ezincinci, ngaphandle kwezixhobo zebhubhoratri.

Ikhithi yePf/Pan Malaria Rapid Test yi-lateral flow chromatographic immunoassay.Amacandelo omcu wovavanyo aquka: 1) i-burgundy colored conjugate pad ene-mouse anti- pHRP-II antibody edityaniswe negolide ye-colloid (i-pHRP II-conjugates yegolide) kunye ne-mouse anti-pLDH ye-antibody edityaniswe negolide ye-colloid (i-pLDH-gold conjugates),

2) i-nitrocellulose membrane strip equkethe iibhendi ezimbini zokuvavanya (i-Pan kunye ne-Pv bands) kunye nebhendi yokulawula (i-C band).I-Pan band ifakwe ngaphambili kunye ne-anti-pLDH ye-anti-monoclonal apho usulelo kunye naluphi na uhlobo lweentlobo ezine ze-plasmodia lunokubonwa, iqela le-Pf lifakwe ngaphambili kunye ne-polyclonal anti-pHRP-II antibodies ukuze kufunyanwe usulelo lwe-Pf, kunye neqela le-C liqatywe ngebhokhwe ye-anti-mouse IgG.

Ngexesha lovavanyo, umthamo owaneleyo wesampuli yegazi unikezelwa kwisampulu kakuhle (S) yekhasethi yovavanyo, i-lysis buffer yongezwa kwi-buffer well (B).Isithinteli sinesicoci esiseza iiseli ezibomvu zegazi kwaye sikhuphe iiantigens ezahlukeneyo zeplasmodium, ezithi zifuduke ngentshukumo yecapillary kumtya ogcinwe kwikhasethi.I-pHRP-II ukuba izipho kumzekelo ziya kubophelela kwi-pHRP II-conjugates yegolide.I-immunocomplex ke ibanjwe kwi-membrane yi-anti-pHRPII anti-coated ngaphambili, eyenza i-burgundy enemibala ye-Pf band, ebonisa isiphumo sovavanyo oluhle lwe-Pf.I-pLDH ukuba izinikezelo kumzekelo ziya kubophelela kwi-pLDH conjugates zegolide.I-immunocomplex ke ibanjwe kwi-membrane yi-anti-coated anti-pLDH antibody, eyenza i-burgundy enemibala ye-Pan band, ebonisa umphumo we-plasmodium positive test.Ngokungabikho kwebhendi yePan, iziphumo zovavanyo ezilungileyo kuyo nayiphi na enye iplasmodia emithathu inokucetyiswa.

Ukungabikho kwazo naziphi na iibhendi zovavanyo (i-Pan kunye ne-Pf) zibonisa isiphumo esibi.Uvavanyo luqulethe ulawulo lwangaphakathi (i-C band) ekufuneka ibonise ibhendi enemibala e-burgundy ye-immunocomplex yebhokhwe anti-mouse IgG / mouse IgG (pHRP-II kunye ne-pLDH-gold conjugates) kungakhathaliseki ukuba ukuphuhliswa kombala kuyo nayiphi na ibhendi yovavanyo. .Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.